TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | Plasmid storage | |
| XL1-Blue | QuikChange transformation strain | Stratagene |
| Xanthomonas sp. | ||
| 35Y | Growth on poly(cis-1,4-isoprene) latex, clearing zone formation | 16 |
| 35Y-CM | Cmr mutant of 35Y | 18 |
| 35Y ΔroxA-attB (SN3727) | Chromosomal deletion of roxA in Xanthomonas sp. 35Y-CM, attB at former roxA site; no clearing zone formation on latex agar | 4 |
| 35Y ΔroxA-attB pNH1-roxA-attP in chromosome (SN4230) | Expression of RoxA from rhamnose promoter in Xanthomonas sp. 35Y-CM; Kmr Cmr; clearing zone formation in the presence of rhamnose plus latex | 4 |
| 35Y ΔroxA-attB pNH1-lcp-attP in chromosome (SN5343) | Expression of Lcp from rhamnose promoter in Xanthomonas sp. 35Y-CM; Kmr Cmr; positive fuchsin staining on latex overlay agar | This study |
| Plasmids | ||
| pMK::lcp (SN5314) | GeneArt vector, source of lcp; Kmr | This study |
| pUC9::lcp (SN5339) | Cloning vector for lcp; Apr | This study |
| pNH1-roxA-attP (SN4230) | Coding sequence of roxA under rhamnose promoter control, attP site; Kmr | 4 |
| pNH1-lcp-attP (SN5341) | Coding sequence of lcp under rhamnose promoter control, attP site; Kmr | This study |
a
Cmr, chloramphenicol resistance; Apr, ampicillin resistance; Kmr, kanamycin resistance.