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Ligand blot analysis of 125I-Cry1Ac (A) and 125I-Cry1Ca (B) toxin binding to BBMV proteins from C. suppressalis (SSB) and S. inferens (PSB). Proteins (20 μg) were separated by SDS–8% PAGE, transferred to a PVDF filter, and then probed with 125I-Cry1Ac or 125I-Cry1Ca toxins. Bound toxin was detected by autoradiography at −80°C. Arrows indicate main BBMV proteins recognized by each toxin in each BBMV sample.
