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Cytograms for untreated (A), conventionally heated (B), or 400-W-DW treated (C) Escherichia coli suspensions stained with PI. The cytogram (A) corresponds to control cell suspension maintained at room temperature. Cell treatments for panels B and C were performed at 37°C during the heating phase (temperature increase from room level up to 37°C). Windows R1, R2, and R3, respectively, corresponded to highly, low-level-, and no PI-stained cells.
