PTS-dependent sugar:phosphotransferase activity. S. mutans UA159 and a manLMN deletion mutant (A) or a manO deletion mutant (B) were grown in TV base medium supplemented with glucose. PTS-dependent transport of glucose (glc), GlcNAc, or GlcN was determined using permeabilized cells as described in Materials and Methods. Each bar represents the average of results of three independent experiments, with error bars indicating the standard deviation. *, P < 0.05 (by the Student t test). ns, not significant.