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. 2014 Aug;13(8):1085–1094. doi: 10.1128/EC.00042-14

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Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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FIG 1 — (A) Scheme of the RcyA protein. The F-box domain, the region with similarity to Sec10, and the CAAX motif are labeled. The original yeast two-hybrid (Y2H) clone is indicated above the scheme. (B) Relatedness analysis of RcyA. Fungal orthologues of RcyA and of Sec10 were aligned by tCoffee, and the tree was plotted at the EBI website (http://www.ebi.ac.uk/Tools/msa/tcoffee/). For the alignment, we used the Rcy1 orthologues of the following organisms: Saccharomyces cerevisiae (Rcy1p) (YJL204C), Saccharomyces paradoxus (spar343-g75.1), Saccharomyces mikatae (smik835-g3.1), Saccharomyces bayanus (sbayc610-g6.1), Candida glabrata (CAGL0F02497g), Saccharomyces castellii (Scas531.3), Kluyveromyces waltii (Kwal33.13585), Kluyveromyces lactis (KLLA0C03300g), Saccharomyces kluyveri (SAKL0C04004g), Ashbya gossypii (AFR644C), Candida lusitaniae (CLUG04919), Debaryomyces hansenii (DEHA2E19052g), Candida guilliermondii (PGUG03406.1), Candida tropicalis1 (CTRG05296.3), Candida tropicalis2 (CTRG06241.3), Candida albicans (orf19.3203), Candida parapsilosis (CPAG03322), Lodderomyces elongisporus (LELG03751), Yarrowia lipolytica (Sls2) (YALI0B19074g), Aspergillus nidulans (RcyA) (AN10061), Neurospora crassa (NCU03658), Schizosaccharomyces japonicus (SJAG02753), Schizosaccharomyces octosporus (SOCG02810), and Schizosaccharomyces pombe (Pof6) (SPCC18.04). In addition, we included the Se10 orthologues of the following organisms (boxed): Saccharomyces cerevisiae (YLR166C), Aspergillus nidulans (AN8879), Schizosaccharomyces pombe (SEC10) (SPAC13F5.06c), and Homo sapiens (hSec10) gi|24418661|sp|O00471.1|.