TABLE 3.
Expression of selected genes involved in secondary metabolism, stress response, and development
| Gene group | Gene or protein name(s) | AFLA gene designation | Ratio of expressiona |
Level of expression (SU-1/3357 YES)b | |
|---|---|---|---|---|---|
| SU1 YES/YEP | 3357 YES/SU-1 YEP | ||||
| Aflatoxin | aflF, norB | 13944 | +>100* | ||
| aflU, cypA | 13943 | +528 | +89 | 2,600, 357; +7.3 | |
| aflT | 13942 | +528 | +89 | 2,600, 357; +7.3 | |
| hypC | 13940 | +528 | +89 | 2,600, 357; +7.3 | |
| aflD, nor-1 | 13939 | +528 | +89 | 2,600, 357; +7.3 | |
| aflA, fas-2 | 13938 | +10 | +8 | ||
| aflB, fas-1 | 13937 | +>10* | |||
| aflR | 13936 | +1.8 | +3 | ||
| aflJ, estA | 13932 | +1,671 | +960 | 664, 288; +2.3 | |
| hypE | 13929 | +7,361 | +960 | ||
| hypD | 13927 | +70 | |||
| aflG, avnA | 13926 | +7,361 | +960 | ||
| aflL, verB | 13925 | +7,361 | +960 | ||
| hypB | 13924 | +7,361 | +960 | ||
| aflI, avfA | 13923 | +7,361 | +960 | ||
| aflO, omtB | 13922 | +7,361 | +960 | ||
| aflP, omtA | 13921 | +>1,000 | +>1,000 | ||
| aflQ, ordA | 13920 | +>1,000 | +>1,000 | 106, 22; +4.8 | |
| aflK, vbs | 13919 | +346 | +100 | ||
| aflV, cypX | 13918 | +>1,000 | +>1,000* | ||
| aflW, moxY | 13917 | +346 | +100 | ||
| aflX, ordB | 13916 | +>1,000* | +>1,000* | ||
| aflY, hypA | 13915 | +>1,000* | +>1,000* | ||
| nadA | 13914 | +346 | +100 | ||
| hxtA | 13913 | −10* | +>100* | ||
| glcA | 13912 | +346 | +100 | ||
| sugR | 13911 | −10* | |||
| Stress response | Mn SOD | 3342 | +129 | +700 | 167, 775; −4.6 |
| Hsp70 | 849 | 11, 24; −2.2 | |||
| Xylulose reductase | 1558 | +2.5 | 132, 282; −2.1 | ||
| Conidiation | Medusa | 13641 | +>100* | ||
| RosA | 2193 | +>100* | |||
| AbaA | 2962 | +3* | |||
| BrlA | 8285 | +2* | +>100* | ||
| Secondary metabolism | AroM | 1768 | +2 | 16, 7; +2.3 | |
| PKS_NRPS (CPA) | 13949 | +>100 | 0.1, 17; −170 | ||
| C6 transcription factors | AflR | 13936 | +3 | ||
| Unknown | 6437 | −3* | −>10* | ||
| Unknown | 4737 | −2* | |||
| Cluster 29 | 8409 | +>10* | +>100 | ||
| Cluster 11 | 2304 | −5 | −10 | ||
| Other transcription factors | AtfB | 9401 | +4 | ||
| Housekeeping genes | Citrate synthase | 4929 | −25 | −40 | |
The ratios of raw expression data of A. parasiticus SU-1 grown for 40 h in YES medium to strain SU-1 grown for 40 h in YEP medium (SU-1 YES/YEP) and A. flavus 3357 grown for 40 h in YES medium to A. parasiticus SU-1 grown for 40 h in YEP medium (3357 YES/SU-1 YEP) are shown. All values reported in these two columns are significant (q < 0.05) except for the values with asterisks. The values with asterisks display trends in differential expression, but they are not statistically significant (q > 0.05). Symbols; +, upregulated in YES or YEP medium; −, downregulated in YES or YEP medium.
The level of expression of A. parasiticus SU-1 grown for 40 h in YES medium and the level of expression of A. flavus 3357 grown for 40 h in YES medium (SU-1/3357 YES) are shown. The first value represents the raw expression data for strain SU-1 grown in YES medium at 40 h, and the second value represents the raw expression data for strain 3357 grown in YES medium for 40 h. The ratio of these values determines the fold upregulation (+) or downregulation (−), which is shown after the first two values. All values reported in this column are statistically significant.