FIG 6.

Development of fungal mycelia in planta. At 7, 14, and 21 dpi, infected leaves were harvested and stained with trypan blue and fungal structures were analyzed under a microscope. Wild-type strain UK7 grows normally and establishes a dense subcuticular stroma. In contrast, the deletion mutant ΔPFP1 can be found in only a very few small lesions, where the fungus starts to develop thick, short-septate hyphae only very late upon inoculation. The complementation mutant 132 also grows slower than the wild type but succeeds with the production of many more symptoms than the deletion mutant. Bars = 50 μm.