FIG 4.

Trypanosomal adenylate cyclases localize to flagellum subdomains. (A) Trypanosomes expressing the indicated HA-tagged adenylate cyclase were subjected to immunofluorescence. Cells were stained with anti-HA antibodies (green), and nuclear and kinetoplast DNA were visualized with DAPI (blue). (B) Immunofluorescence analysis as described for panel A showing dividing cells. Cells have a single nucleus and two kinetoplasts, indicating that they have not completed mitosis, and possess a mature flagellum (filled arrowhead) as well as a newly forming daughter flagellum (unfilled arrowhead).