FIG 6.

C-terminal sequences are required for targeting to the flagellum. (A) Western blot analysis of whole-cell lysates from cells expressing the indicated HA-tagged protein. Blots were probed with anti-HA or anti-tubulin antibodies. (B) Trypanosomes expressing the indicated HA-tagged deletion mutants were subjected to immunofluorescence. Cells were stained with anti-HA antibodies (green) and anti-PFR antibodies (red). Nuclear and kinetoplast DNA were visualized with DAPI (blue). (C) (Top) Schematic diagram illustrating amino acid sequence identities between ACP1 and ACP2, which are nearly identical except for a short region at the C terminus. (Bottom) Alignment of the C termini of ACP1 to ACP6 (P1 to P6). ACP1 residues that differ from ACP2 are boxed, and among these, those residues that are conserved between ACP1 and ACP4 are highlighted in gray.