FIG 2.

HCV replicon elimination with DCV. Huh-7 cells harboring GT-1 to -6 replicons were treated with the indicated concentrations of DCV for 14 days in the absence of G418. Cell cultures were maintained at subconfluency by splitting as described in Materials and Methods. After 14 days, DCV was removed, and a portion of the cell cultures were treated with medium containing G418 (500 μg/ml) for 2 weeks to allow cells that harbored the HCV replicon to form colonies. The colonies that formed were photographed. For GT-2a JFH, GT-2a M31, and GT-3a replicon cells, parallel cultures of cells were amplified for genotypic analysis. The replicon cell lines used in this study are as follows: 1b, Con1, and 1a, H77c (2, 13); 2a JFH, JFH-1 (AB047639); 2a M31 NS5A (aa 1 to 425), 2a infectious clone-pJ6CF (NC_009823.1); 3a NS5A (aa 1 to 429), HCV3a1 (JX944789); 4a NS5A, HCV4a-23 (JQ347515); 5a NS5A (aa 1 to 430), GT-5a-7 (KJ719453); and 6a NS5A (aa 1 to 431), GT-6a-16 (KJ719455).