TABLE 1.

DCV activity on parental and selected GT-5a NS5A hybrid replicon cell lines

Replicon	EC50 (nM) for parental cellsa	DCV selection concn (nM)b	EC50 (nM) for selected cellsa	NS5A amino acid substitution(s) (%)c
GT-5a-6	0.0072 ± 0.0007	Not done	NAd	NA
GT-5a-7	0.0036 ± 0.0007	DMSO	0.0054 ± 0.0002	None
		1	14 ± 0.22	L31F/V (80/20)
		10	37 ± 0.51	L31F (100), K56R (50)
		100	No cells survived	NA
GT-5a-9	0.003 ± 0.0003	DMSO	0.0042 ± 0.0006	None
		1	4.5 ± 0.35	L31F/V (45/55)
		10	No cells survived	NA
		100	No cells survived	NA

^aValues are means ± standard deviations (n ≥ 3).

^bAn equivalent percent volume of DMSO alone was used as a control.

^cSubstitutions were deduced from bulk NS5A sequence traces. Numbers in parentheses represent the estimated percentages of the indicated amino acids.

^dNA, not applicable.