TABLE 4.
DCV activity on the parental and selected GT-6a NS5A hybrid replicon (GT-6a-16) cell lines
| Replicon | EC50 (nM) for parental cellsa | DCV selection concn (nM)b | EC50 (nM) for selected cellsa | NS5A amino acid substitution(s) (%)c |
|---|---|---|---|---|
| GT-6a-16 parent | 0.074 ± 0.005 | DMSO | 0.041 ± 0.004 | None |
| GT-6a-16 DCV selected | 1 | 6.4 ± 1.3 | P32S (25), T58A/S (45/10) | |
| 10 | 59.2 ± 8.2 | L31 M (20), P32L/S (10/70) | ||
| 100 | 323 ± 6.5 | P32L (100) |
a
Values are means ± standard deviations (n ≥ 3).
b
An equivalent percent volume of DMSO alone was used as a control.
c
Substitutions were deduced from bulk NS5A sequence traces. Numbers in parentheses represent the estimated percentages of the indicated amino acids.