TABLE 2.

In vitro antifungal susceptibility profile of H. capsulatum isolates reported globally

Location of isolation by continent	Yr of isolation	No. investigated (+ no. of reference strains investigated) (dimorphic form)a	Method used (source)	MIC/MIC range (μg/ml) forb:								Reference or source
				AMB	ITC	VRC	POS	ISA	FLU	CAS/MFG/AFGc	RAV
North America
St. Louis, MO	1990	8 + 4 (Y)	Broth microdilution using SDBd	0.30 to 1.04					2.95 to >1,000			12
Indiana	1997	1 (Y)	Broth macrodilution (NCCLS)		0.004				0.62 (parent), 1.25 (8th wk), 2.5 (12th wk), 20 (16th wk)			14
Virginia	1998	5 (M)	Broth microdilution (NCCLS)	0.25 to 0.5	0.06	0.06						15
Virginia	1998	5 (M)	Broth microdilution (NCCLS)				0.04			CAS, 1.3; AFG, 3.6		16
Indiana	2000	20 (Y)	Modified broth microdilution (NCCLS M-27A)	0.5 to 1						8 to 32		11
Texas	2000	100 (M)	Modified broth microdilution (NCCLS M-27A)	<0.03 to 2	<0.03 to 0.5	<0.03 to 2						17
Indiana	2001	65 (M)	Modified broth microdilution (NCCLS M-27A)		0.019 to 0.077				0.31 to 10			8
Virginia	2003	4–5 (M)	Broth macrodilution (NCCLS M38-A)	0.06 to 0.5	<0.01 to 0.03		<0.03 to 0.06		1 to 16	CAS, 0.5 to 4; AFG, 2 to 4; MFG, >0.03 to 0.06		18
Mexico	2005	28 (M)	Broth macrodilution (NCCLS M38-A)		0.06 to 1	0.06 to 2	0.03 to 2		2 to 32		0.125 to 2	19
Indiana	2006	17 (median MIC) (Y)	Broth microdilution (NCCLS M-27A)			0.015	0.007		1		0.007	20
Mexico	2009	28 (M)	Broth macrodilution (CLSI M38-A)	0.06 to 0.25	0.25 to 2	0.06 to 2	0.03 to 2	0.125 to 2	4 to 32			9
South America
Brazil	2012	68 (M)	Modified broth microdilution (NCCLS M38-A)	0.007 to 0.5	0.001 to 0.031	0.0078 to 0.5			3.9 to 125	0.016 to 32		21
		8 (Y)	Modified broth microdilution (CLSI M27-A2)	0.06 to 0.5	0.0039 to 0.03	0.002 to 0.03			3.9 to 7.8	1 to 4
Asia
Japan	2010	3 (M)	Modified broth microdilution (CLSI M38-A)	0.013 to 0.05	<0.0004	0.006 to 0.025		0.003 to 0.006	0.55 to 1.2			22
India	2014	21 + 2 (M)	Modified broth microdilution (CLSI M38-A2)	0.03 to 0.25	<0.03 to 0.125	<0.03 to 0.25	0.015 to 0.125	0.015 to 0.25	2 to 32	0.015 to 0.5		Present study
		21 + 2 (Y)	Modified broth microdilution (CLSI M27-A3)	0.03 to 0.5	0.03 to 0.25	0.03 to 0.5	0.03 to 0.5	0.015 to 0.125	2 to 8	0.03 to 1

^aM, mycelial form; Y, yeast form.

^bAMB, amphotericin B; ITC, itraconazole; VRC, voriconazole; POS, posaconazole; ISA, isavuconazole; FLU, fluconazole; CAS, caspofungin; RAV, ravuconazole; MFG, micafungin; AFG, anidulafungin.

^cFor caspofungin, MEC were defined for testing the mycelial form.

^dSDB, Sabouraud's dextrose broth.