TABLE 1.
Bacterial strains and plasmids used in this study
| S. aureus strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| Mu3 | hVISA clinical isolate from JUH in 1996, carrying vraS(I5N), stably forms large-sized colonies | 1 |
| Mu50 | VISA clinical isolate from JUH in 1996, carrying vraS(I5N) and rpoB(H481Y) | 1 |
| 6R-P | Mutant isolated from Mu3 by selection with 6 mg/liter vancomycin; spontaneously generates LC and SC | This study |
| L1 | Derivative of 6R-P with large colony morphology | This study |
| L2 | Derivative of 6R-P with large colony morphology | This study |
| L3 | Derivative of 6R-P with large colony morphology | This study |
| N315ΔIP (= ΔIP) | mecI-null mutant (mecI::tetL) of N315P, an N315-derivative strain cured of PCase plasmid | 41 |
| ΔIPrpoB(R512P) | ΔIP introduced with rpoB(R512P) mutation by allelic replacement | This study |
| H14 (= ΔIPvraS(S329L) | Mutant derived from ΔIP by selection with 8 mg/liter imipenem, carrying vraS(S329L) mutation | 10 |
| H14rpoB(R512P) | H14 introduced with rpoB(R512P) mutation by allelic replacement | This study |
| Plasmids | ||
| pKOR1 | E. coli-S. aureus shuttle vector for construction of allelic replacement | 8 |
| pKOR1-rpoB(512P) | pKOR1 harboring the 1,884-bp PCR product of the rpoB gene encompassing its rpoB(R512P) mutation | This study |
| pKOR1-vraS(329L) | pKOR1 harboring the 1,000-bp PCR product of the vraS gene encompassing its vraS(S329L) mutation | 10 |
a
JUH, Juntendo University Hospital. The strains obtained by antibiotic selection are denoted as mutants.