TABLE 5.
Introduction of rpoB(R512P) mutation causes thickening of cell wall observed with transmission electron microscopy
| Strain | Phenotype | Cell wall thickness parameters (nm) |
|
|---|---|---|---|
| Drug-free measurement (mean ± SD) | Ratioa | ||
| Mu3 | hVISA | 21.5 ± 2.4 | 1 (ref) |
| Mu50 | VISA | 32.7 ± 3.0 | 1.52 |
| 6R-P | sVISA | 26.1 ± 2.6 | 1.21 |
| L1 | hVISA | 23.8 ± 2.0 | 1.11 |
| L2 | hVISA | 22.8 ± 1.8 | 1.06 |
| L3 | hVISA | 20.7 ± 1.9 | 0.96 |
| ΔIP | VSSA | 16.4 ± 2.9 | 1 (ref) |
| ΔIPrpoB(R512P) | hVISA | 26.8 ± 3.4 | 1.63 |
| H14 [= ΔIPvraS(S329L)] | hVISA | 26.1 ± 4.3 | 1 (ref) |
| H14rpoB(R512P) | sVISA | 27.3 ± 2.4 | 1.05 |
a
Relative cell wall thickness compared to those of the reference strains Mu3, ΔIP, and H14. ref, reference thickness.