FIG 3.
GaIII inhibitory activity and intracellular GaIII levels in P. aeruginosa PAO1 and isogenic PCH-defective mutants. (A) MICs of Ga(NO3)3 (μM) for P. aeruginosa PAO1 and isogenic PCH-transport mutants grown for 24 h and 48 h in 200 μl DCAA supplemented with increasing Ga(NO3)3 concentrations (0 to 100 μM). (B) Intracellular concentrations of GaIII in the same P. aeruginosa strains grown in DCAA supplemented with 3 μM Ga(NO3)3 for 14 h, measured by the means of ICP-OES. The values are expressed as the percentage relative to PAO1 and represent the mean ± standard deviation from three independent experiments. Intracellular GaIII levels in wild-type PAO1 varied from 0.47 to 1.41 ng GaIII/mg total cell proteins, depending on the experiment. *, statistically significant differences relative to PAO1 (P < 0.05, ANOVA).