TABLE 2.
Biofilm-inhibitory activity of truncated forms of P318
| Peptide | Sequence | BIC-2 (μM) ± SEM | BIC-5 (μM) ± SEM |
|---|---|---|---|
| P318 | [H]KIGEKLKKIGQKIKNFFQKLVPQPEQ[NH2] | 0.15 ± 0.03 | 0.32 ± 0.04 |
| 318_1 | [H]IGEKLKKIGQKIKNFFQKLVPQPEQ[NH2] | 0.33 ± 0.10 | 7.83 ± 1.86 |
| 318_2 | [H]GEKLKKIGQKIKNFFQKLVPQPEQ[NH2] | 0.20 ± 0.04 | 0.43 ± 0.07 |
| 318_3 | [H]EKLKKIGQKIKNFFQKLVPQPEQ[NH2] | 0.25 ± 0.04 | 0.33 ± 0.01 |
| 318_4 | [H]KLKKIGQKIKNFFQKLVPQPEQ[NH2] | >25 | >25 |
| 318_5 | [H]LKKIGQKIKNFFQKLVPQPEQ[NH2] | 0.21 ± 0.01 | 0.30 ± 0.00 |
| 318_6 | [H]KKIGQKIKNFFQKLVPQPEQ[NH2] | 0.25 ± 0.03 | 0.40 ± 0.03 |
| 318_7 | [H]KIGQKIKNFFQKLVPQPEQ[NH2] | 0.24 ± 0.02 | 0.35 ± 0.02 |
| 318_8 | [H]IGQKIKNFFQKLVPQPEQ[NH2] | 0.30 ± 0.04 | 0.46 ± 0.06 |
| 318_9 | [H]GQKIKNFFQKLVPQPEQ[NH2] | 0.43 ± 0.13 | 0.58 ± 0.14 |
| 318_10 | [H]QKIKNFFQKLVPQPEQ[NH2] | 1.50 ± 0.50 | 10.30 ± 3.90 |
| 318_11 | [H]KIKNFFQKLVPQPEQ[NH2] | 0.30 ± 0.04 | 1.74 ± 0.73 |
| 318_12 | [H]IKNFFQKLVPQPEQ[NH2] | 0.78 ± 0.25 | 4.50 ± 1.50 |
| 318_13 | [H]KNFFQKLVPQPEQ[NH2] | 8.37 ± 4.52 | 18.33 ± 1.67 |
| 318_14 | [H]NFFQKLVPQPEQ[NH2] | 8.40 ± 4.18 | 20.00 ± 5.00 |
| 318_15 | [H]FFQKLVPQPEQ[NH2] | 12.00 ± 4.16 | >25 |
| 318_16 | [H]FQKLVPQPEQ[NH2] | >25 | >25 |
| 318_17 | [H]QKLVPQPEQ[NH2] | >25 | >25 |
| 318_18 | [H]KLVPQPEQ[NH2] | >25 | >25 |
| 318_19 | [H]LVPQPEQ[NH2] | >25 | >25 |
| 318_20 | [H]VPQPEQ[NH2] | >25 | >25 |
| 318_21 | [H]PQPEQ[NH2] | >25 | >25 |
| 318_22 | [H]QPEQ[NH2] | >25 | >25 |
| 318_23 | [H]PEQ[NH2] | 18.75 ± 4.27 | >25 |
| 318_24 | [H]KIGEKLKKIGQKIKNFFQKLVPQPE[OH] | 0.33 ± 0.03 | 0.55 ± 0.09 |
| 318_25 | [H]KIGEKLKKIGQKIKNFFQKLVPQP[OH] | 0.40 ± 0.07 | 0.60 ± 0.15 |
| 318_26 | [H]KIGEKLKKIGQKIKNFFQKLVPQ[OH] | 0.25 ± 0.04 | 0.48 ± 0.14 |
| 318_27 | [H]KIGEKLKKIGQKIKNFFQKLVP[OH] | 0.28 ± 0.02 | 0.31 ± 0.04 |
| 318_28 | [H]KIGEKLKKIGQKIKNFFQKLV[OH] | 10.90 ± 2.34 | 13.75 ± 3.06 |
| 318_29 | [H]KIGEKLKKIGQKIKNFFQKL[OH] | 0.28 ± 0.04 | 0.38 ± 0.01 |
| 318_30 | [H]KIGEKLKKIGQKIKNFFQK[OH] | 0.30 ± 0.00 | 1.70 ± 0.30 |
| 318_31 | [H]KIGEKLKKIGQKIKNFFQ[OH] | 0.23 ± 0.05 | 0.47 ± 0.06 |
| 318_32 | [H]KIGEKLKKIGQKIKNFF[OH] | 0.22 ± 0.03 | 0.40 ± 0.00 |
| 318_33 | [H]KIGEKLKKIGQKIKNF[OH] | 0.28 ± 0.03 | 0.59 ± 0.12 |
| 318_34 | [H]KIGEKLKKIGQKIKN[OH] | 0.29 ± 0.02 | 0.53 ± 0.16 |
| 318_35 | [H]KIGEKLKKIGQKIK[OH] | 0.30 ± 0.00 | >25 |
| 318_36 | [H]KIGEKLKKIGQKI[OH] | 0.44 ± 0.09 | 0.70 ± 0.10 |
| 318_37 | [H]KIGEKLKKIGQK[OH] | 0.36 ± 0.08 | 0.80 ± 0.00 |
| 318_38 | [H]KIGEKLKKIGQ[OH] | 3.50 ± 1.00 | 5.83 ± 0.17 |
| 318_39 | [H]KIGEKLKKIG[OH] | 4.17 ± 3.71 | 6.00 ± 0.00 |
| 318_40 | [H]KIGEKLKKI[OH] | 4.00 ± 1.15 | 8.00 ± 1.50 |
| 318_41 | [H]KIGEKLKK[OH] | 12.38 ± 4.32 | >25 |
| 318_42 | [H]KIGEKLK[OH] | >25 | >25 |
| 318_43 | [H]KIGEKL[OH] | >25 | >25 |
| 318_44 | [H]KIGEK[OH] | >25 | >25 |
| 318_45 | [H]KIGE[OH] | >25 | >25 |
| 318_46 | [H]KIG[OH] | >25 | >25 |
| 318_47 | [H]IGEKLKKIGQKIKNFFQKLVPQPE[OH] | 0.28 ± 0.02 | 0.33 ± 0.03 |
| 318_48 | [H]GEKLKKIGQKIKNFFQKLVPQP[OH] | 0.25 ± 0.03 | 0.45 ± 0.05 |
| 318_49 | [H]EKLKKIGQKIKNFFQKLVPQ[OH] | 0.25 ± 0.05 | 0.28 ± 0.05 |
| 318_50 | [H]KLKKIGQKIKNFFQKLVP[OH] | 0.24 ± 0.06 | 0.38 ± 0.08 |
| 318_51 | [H]LKKIGQKIKNFFQKLV[OH] | >25 | >25 |