TABLE 4.

Synergistic activity of AS10 with caspofungin or amphotericin B against C. albicans biofilms^a

AS10 concn (μM)	Caspofungin plus AS10				Amphotericin B plus AS10				Caspofungin plus AS10				Amphotericin B plus AS10
	BEC-2 CAS (μM) ± SEM	P value	Fold change	FICI	BEC-2 AmB (μM) ± SEM	P value	Fold change	FICI	BEC-5 CAS (μM) ± SEM	P value	Fold change	FICI	BEC-5 AmB (μM) ± SEM	P value	Fold change	FICI
0	0.65 ± 0.17	NA	NA	NA	0.67 ± 0.17	NA	NA	NA	0.70 ± 0.10	NA	NA	NA	1.23 ± 0.28	NA	NA	NA
1.56	0.08 ± 0.03	0.005	8.63	0.120	0.13 ± 0.02	0.007	5.03	0.203	0.09 ± 0.01	0.003	8.00	0.129	0.24 ± 0.03	0.004	5.14	0.171
0.78	0.09 ± 0.03	0.006	7.40	0.137	0.12 ± 0.01	0.006	5.58	0.181	0.12 ± 0.00	0.004	5.83	0.173	0.21 ± 0.04	0.003	5.97	0.158
0.39	0.15 ± 0.01	0.011	4.26	0.236	0.29 ± 0.02	0.043	2.34	0.429	0.28 ± 0.01	0.013	2.55	0.394	0.50 ± 0.03	0.021	2.48	0.245

^aAmB, amphotericin B; CAS, caspofungin; NA, not applicable. One-way ANOVA with Bonferroni's multiple-comparison test was used to compare the BEC-2 and BEC-5 of CAS and AMB in the absence of AS10 with their BEC-2 and BEC-5 in the presence of the indicated AS10 concentrations.