FIG 8.

Effect of CQ on HEK293T cell line. HEK293T cells were grown as described in Materials and Methods. Equal numbers of cells were treated with increasing concentrations of CQ for 30 min. Protein extracts were made, and Western blotting was performed with anti-P38 phosphoantibody (A) or anti-P42/44 (B) phosphoantibody. NaCl treated cells were used as a positive control for P38 phosphorylation. A nonspecific band appearing with P38 or P42/44 antibody and a Ponceau S-stained image of representative Western blots were used as loading controls.