FIG 1.
The effect of vanYn gene dosage and vanHatAatXat heterologous expression on the growth of Nonomuraea sp. The cells were grown in VSP medium, where production of A40926 was not detected by HPLC (limit of detection, 2 mg/liter). ■, Nonomuraea sp. ATCC 39727 wild type; ○, Nonomuraea sp. strain carrying pSET152Y; ●, Nonomuraea sp. strain carrying pRT802Y; ◇, Nonomuraea sp. ΔvanYn-pRT802Y strain; △, Nonomuraea sp. strain carrying pIJ86Y; ◆, Nonomuraea sp. strain carrying pST30. The values represent the averages and standard deviations from three independent experiments, with a standard deviation of <5%.