FIG 4.
Growth, resistance, and A40926 production in Nonomuraea sp. ATCC 39727 and its derivatives. The Nonomuraea spp. strains listed in Table 1 were grown in FM2 medium in 2-liter bioreactors. Growth parameters (glucose consumption, ■; dry weight, ●), levels of A40926 production (gray bars), the MIC of A40926 (○), and VanYn activity (black bars) were measured as described in Materials and Methods in Nonomuraea sp. ATCC 39727 (A), Nonomuraea sp. strain carrying pSET152Y (B), Nonomuraea sp. strain carrying pRT802Y (C), the Nonomuraea sp. ΔvanYn-pRT802Y strain (D), Nonomuraea sp. strain carrying pIJ86Y (E), and Nonomuraea sp. strain carrying pST30 (F). Data represent the averages from three independent experiments, and error bars indicate the standard deviation.