TABLE 3.
MICs of glycopeptides for Nonomuraea sp. ATCC 39727 and its recombinant strains, measured in the presence or absence of 50 μg/ml penicillin G
| Nonomuraea sp. strain genotype or plasmid | MIC (μg/ml) fora: |
|||||
|---|---|---|---|---|---|---|
| VAN | VAN + PENb | TEC | TEC + PENb | A40 | A40 + PENb | |
| Wild type | 30 | 17.5 | 0.9 | 0.5 | 4 | 3.5 |
| pSET152 | 30 | 17.5 | 0.9 | 0.5 | 4 | 3.5 |
| pSET152Y | 40 | 30 | 2 | 1.5 | 15 | 4 |
| pRT802 | 30 | 17.5 | 0.9 | 0.5 | 4 | 3.5 |
| pRT802Y | 30 | 20 | 1.25 | 0.75 | 15 | 10 |
| pIJ86 | 30 | 17.5 | 1 | 0.6 | 5 | 3.5 |
| pIJ86Y | 25 | 20 | 1.25 | 1 | 15 | 10 |
| ΔvanYn | 4 | 4 | 0.2 | 0.2 | 2 | 2 |
| ΔvanYn-pRT802 | 4 | 4 | 0.2 | 0.2 | 2 | 2 |
| ΔvanYn-pRT802Y | 25 | 15 | 1.25 | 0.5 | 15 | 10 |
| pST30 | 15 | 15 | 2.75 | 2.75 | 17.5 | 17.5 |
a
MICs were determined using strains grown in VSP medium for 72 h and the agar dilution method (21). The values represent the average of the data from three independent experiments. VAN, vancomycin; TEC, teicoplanin; A40, A40926; PEN, penicillin.
b
Penicillin G was added to the agar medium together with the different concentrations of glycopeptides.