FIG 3.

RI20 inhibits repression of OmpF by Hfq-RybB and Hfq-MicF. (A) With no inhibitor, RybB represses OmpF′-yfp expression through base-pairing interactions (top), and E. coli pRybB pompF′-yfp cells have low YPF fluorescence intensity (RybB panel). When RI20 is produced (RybB + pRI20 panel), fluorescence intensity is higher. A strain with no RybB expression (E. coli pompF′-yfp [no RybB panel]) is shown as a positive control. (B) OmpF is also repressed through base-pairing interaction with MicF (top), and E. coli pMicF pompF′-yfp cells have low YPF fluorescence intensity (MicF panel). Production of RI20 increases YFP fluorescence intensity (MicF + pRI20 panel). A strain with no MicF expression (E. coli pompF′-yfp [no MicF panel]) is shown as a positive control.