FIG 5.
RI20 inhibits Hfq binding to MicF in vitro. Gel mobility shift assays were used to measure the affinity of Hfq and MicF in the presence of different concentrations of RI20. Representative gels with (A) no RI20 and (B) with 500 μM RI20 are shown. For each gel, the fraction bound was plotted and nonlinear regression was used to determine the apparent Kd. (C) Representative curves at each RI20 concentration (0 [filled circles], 50 [squares], 100 [open triangles], 300 [filled triangles], and 500 [open circles] μM) are shown. (D) The average Kd app values for at least three repeats at each concentration were plotted versus RI20 concentration to determine the Ki. Whiskers indicate the standard deviation at each point.