TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| BW27786 | DE(araBAD) DE(rhaBAD) DE(araFGH) ϕ(ΔaraEp PCP13-araE ΔrhaD-B) | 24 |
| SAE006 | BW27786 lacIq | This study |
| SAE008 | SAE006 pRybB pompC′-yfp | This study |
| SAE009 | SAE006 pJV300 pompC′-yfp | This study |
| SAE011 | SAE006 pMicF pompF′-yfp | This study |
| SAE012 | SAE006 pJV300 pompF′-yfp | This study |
| SAE109 | BL21(DE3) pET28a_hfq | This study |
| SAE204 | SAE011 pRI20 | This study |
| SAE221 | SAE008 pRI20_1 | This study |
| SAE222 | SAE008 pRI20_2 | This study |
| SAE223 | SAE008 pRI20_3 | This study |
| SAE224 | SAE008 pRI20_4 | This study |
| SAE225 | SAE008 pRI20_5 | This study |
| SAE229 | BW27786 Δhfq | This study |
| SAE232 | BW27786 pRI20 | This study |
| SAE250 | SAE006 pRybB pompF′-yfp | This study |
| SAE251 | SAE006 pJV300 pompF′-yfp | This study |
| SAE252 | SAE250 pRI20 | This study |
| pSB4K5 | BioBrick vector, Kanr | 46 |
| pBAD18K | pBAD vector, Kanr | 47 |
| pAS08 | R6K ori plasmid carrying tet control region with tetR, TetR-regulated promoter, and tetA, Cmr | Mark Goulian (University of Pennsylvania) |
| pRybB | Same as pFM1-1 (pBRpLac encoding rybB, Ampr) | 48 |
| pMicF | pBRpLac vector encoding micF, Ampr | 49 |
| pKMT5 | pNM12 vector encoding rybB, Ampr | 50 |
| pJV300 | sRNA control vector, Ampr | 7 |
| pARCBD | Expression plasmid for SICLOPPS, Cmr | 22 |
| pompC′-yfp | pSB4K5 with ompC′-yfp, Kanr | This study |
| pompF′-yfp | pSB4K5 with ompF′-yfp, Kanr | This study |
| pRI20 | pARCBD with RI20 (SGWKVMEG), Cmr | This study |
| pRI20_1 | pARCBD with SGWAVMEG, Cmr | This study |
| pRI20_2 | pARCBD with SGWKAMEG, Cmr | This study |
| pRI20_3 | pARCBD with SGWKVAEG, Cmr | This study |
| pRI20_4 | pARCBD with SGWKVMAG, Cmr | This study |
| pRI20_5 | pARCBD with SGWKVMEA, Cmr | This study |
| pET28a_hfq | pET28a encoding untagged hfq, Kanr | This study |