TABLE 4.
Pairwise comparison of antibody detection-based tests for JD and Ziehl-Neelsen staining, qPCR, and bacteriological culture of gut tissues collected from 41 fallow deer included in the study
| Test combination | No. of deer tested positive/no. total deer by ELISA: |
||||
|---|---|---|---|---|---|
| A | B | C | D | E | |
| ELISA+/ZN+ | 7/8 | 8/8 | 8/8 | 7/8 | 6/8 |
| ELISA+/ZN− | 11/33 | 8/33 | 10/33 | 6/33 | 14/33 |
| Total | 18/41 | 16/41 | 18/41 | 13/41 | 20/41 |
| Kappaa | 0.3676 | 0.5495 | 0.4730 | 0.5605 | 0.2077 |
| P value | 0.0133b | 0.0001b | 0.0004b | 0.0005b | 0.1300 |
| ELISA+/qPCR+ | 5/6 | 5/6 | 5/6 | 4/6 | 5/6 |
| ELISA+/qPCR− | 13/21 | 11/21 | 13/21 | 9/21 | 15/21 |
| Total | 18/27 | 16/27 | 18/27 | 13/27 | 20/27 |
| Kappa | 0.1250 | 0.1940 | 0.1250 | 0.1681 | 0.0649 |
| P value | 0.6279 | 0.3497 | 0.6279 | 0.3845 | 1.0000 |
| ELISA+/culture+ | 1/1 | 1/1 | 1/1 | 1/1 | 1/1 |
| ELISA+/culture− | 17/40 | 15/40 | 17/40 | 12/40 | 19/40 |
| Total | 18/41 | 16/41 | 18/41 | 13/41 | 20/41 |
| Kappa | 0.0619 | 0.0752 | 0.0619 | 0.1022 | 0.0512 |
| P value | 0.4390 | 0.3902 | 0.4390 | 0.3171 | 0.4978 |
a
Kappa is the coefficient of agreement between each pair of diagnostic test results.
b
Statistically significant association between tests (Fisher's test).