FIG 4.

Serum FMDV-specific IgG titers. Mice (n = 8/group) were s.c. immunized with FMDV antigen plus physiological saline, RO containing GS-R (2, 4, or 6 μg), or ISA 206 on days 1 and 21. Sera were collected 2 weeks after boosting, and serum FMDV-specific IgG levels were measured by an indirect ELISA. Values above the cutoff background level (the mean from unimmunized mice as negative controls multiplied by a factor of 2.1) were considered positive. Titers are depicted as reciprocal end dilutions. Bars with different letters have statistically significant differences (P < 0.05).