TABLE 1.
Characteristics of N1-VHHm binding to purified recombinant N1rec as determined by ELISA and SPR
| N1-VHHm | N1rec bindinga | N1rec inhibition | Kon (M−1 s−1) | Koff (s−1) | KD (M) |
|---|---|---|---|---|---|
| 1 | + | + | NDb | ND | ND |
| 2 | + | − | ND | ND | ND |
| 3 | + | + | 3.6 E+6 | 1.3 E−3 | 3.7 E−10 |
| 4 | + | − | ND | ND | ND |
| 5 | + | + | 1.2 E−5 | 5.8 E−4 | 4.7 E−9 |
| 6 | + | + | ND | ND | ND |
| 7 | + | − | 4.4 E+4 | 2.8 E−3 | 6.3 E−8 |
| 8 | + | − | ND | ND | ND |
| 9 | + | − | ND | ND | ND |
| 10 | + | − | ND | ND | ND |
| 11 | − | − | ND | ND | ND |
| 12 | + | − | ND | ND | ND |
| 13 | − | − | ND | ND | ND |
a
Binding of N1rec (recombinant tetrameric N1 NA) was determined by ELISA. A minus sign indicates binding to the tetramerizing zipper and to N1rec.
b
ND, not done.