FIG 5.

eIF4B knockdown transgenic mice are more susceptible to influenza A virus. (A) Shown is the body weight change of wild-type (WT) and eIF4B knockdown mice (TG) mock infected or infected intranasally with WSN. Body weight was measured daily. The results are shown as mean percentage weight changes from three independent experiments. The dashed line indicates 75% of initial body weight. (B) Survival rate of wild-type mice (n = 14) and eIF4B knockdown mice (n = 14) infected intranasally with WSN. Mice were monitored for up to 12 days. During that period, mice were sacrificed when showing hind limb paralysis. *, P < 0.05 (Kaplan-Meier test). (C) Shown is the viral load in the lungs of wild-type and eIF4B knockdown mice infected for 3 days with WSN. Each symbol represents an individual mouse. **, P < 0.01 (Student's t test). (D) Representative micrographs (magnification, ×400) of the wild-type and eIF4B knockdown mouse lungs stained with hematoxylin and eosin (HE). The pathological changes of lungs in TG mice were more serious than in their WT counterparts after infection for 3 days.