FIG 1.

UUKV siRNA screens. (A) Outlines of screening and bioinformatics procedures. (B) Z* score distribution of infection indices obtained for each siRNA in the Qiagen screen regarding inhibitor candidate genes. A redundant siRNA activity (RSA) P value below 0.01 defined potential hits. (C) Z* score distribution, as described for panel B but for enhancer candidate genes. An RSA P value below 0.001 defined potential hits. (D) Dual flash plot for Dharmacon siRNAs, showing selected candidates based on SSMD criteria and the fold change. Inhibitor genes were defined by SSMD and change values below −3-fold and 0.5-fold, respectively, and enhancers were defined as those with values above 2-fold and 1.5-fold (indicated by the gray horizontal lines). (E) Overlaps of candidate genes between the two siRNA screens. The numbers of potential hits that were specific to the Dharmacon and Qiagen screens are indicated in the top and bottom circles, respectively. The total numbers of potential hits for the Dharmacon and Qiagen screens are indicated next to the top and bottom circles, respectively. The P values, based on Fisher's exact statistical tests, indicate that potential hits obtained for both libraries were not independent.