FIG 4.

HPV E7 interacts with Nbs1 and Rad50 but not Mre11. (A) U20S cells were transiently transfected with vector alone (EV), HA-HPV31 E7, and TAP-HPV16 E7, and immunoprecipitations of whole-cell lysates were performed using an HA antibody, followed by immunoblotting with Mre11, Rad50, and Nbs1 antibodies. (B) U20S cells were transiently transfected with EV alone, HA-HPV31 E7, or TAP-HPV16 E7. Immunoprecipitations were performed on lysates using an antibody to Nbs1, followed by Western blotting using antibodies to Mre11, Rad50, and Nbs1 antibodies. For panels A and B, input lysates were analyzed by Western blotting using antibodies to HA, Mre11, Rad50, and Nbs1. GAPDH served as a loading control. IP, immunoprecipitation; *, antibody heavy chain. All results are representative of observations from three independent experiments.