FIG 5.

Depletion of USP1, USP12, USP46, or UAF1 by shRNAs impairs cellular proliferation. (A) Colony formation assay. C33A cells were transfected with the indicated shRNA expression vectors and selected for approximately 3 weeks in puromycin-containing medium. Drug-resistant colonies were fixed with methanol and stained with methylene blue. (B) Cell cycle analysis. C33A cells transiently expressing the indicated shRNA were trypsinized at 72 h posttransfection, and their DNA was stained with Hoechst and analyzed by flow cytometry.