FIG 7.

Specific inhibition of HPV DNA replication by catalytically inactive USP1, USP12, and USP46. (A) HPV DNA replication levels in cells expressing increasing amounts of RFP-tagged USP1ci, USP12ci, and USP46ci (8.75, 37.7, and 75 ng of the RFP-USPci expression vector). DNA replication activities are reported as a percentage of the signal obtained with cells cotransfected with the empty RFP vector as a control (−). Each value is the average of at least three independent experiments, each performed in triplicate, with the standard deviations presented as error bars. (B) The same as for panel A but using a firefly luciferase reporter plasmid lacking the viral origin. (C) Effect of the RFP-USPci enzymes on BPV1 DNA replication. The experiment was performed as described for panel A but using a BPV1 DNA replication assay.