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. 2014 Aug;88(15):8545–8555. doi: 10.1128/JVI.00379-14

FIG 8.

FIG 8

Inhibition of HPV DNA replication by catalytically inactive USP1, USP12, and USP46 requires the UAF1-binding site in E1. (A) Schematic representation of the HPV31 E1 wild-type protein (E1 WT) and of the truncated E1 derivative (E1Δ) lacking the N-terminal 40 amino-acid-long UAF1-binding site (hatched box). The relative DNA replication activity of these two proteins is indicated on the right. (B to D) Effect of overproducing RFP-tagged USP1ci (B), RFP-USP12ci (C), and RFP-USP46ci (D) on HPV DNA replication catalyzed by either the E1 wild-type protein or E1Δ. Increasing amounts of RFP-USPci expression vectors (8.75, 37.7, and 75 ng) were used. DNA replication activities are reported as a percentage of the signal obtained with cells cotransfected with the empty RFP vector as a control (−). Each value is the average of three independent experiments, each performed in triplicate, with the standard deviations presented as error bars.