TABLE 1.
The position and nucleotide changes introduced into the antigenome cDNA clones of NDV strains BC and GBT
| Location | Nucleotide change(s)a | RE site | Change to ORFe |
|---|---|---|---|
| N gene 5′-NTRb | A(1768)T, C(1769)T, C(1773)T | PacIc | None |
| P gene 5′-NTRb | A(3221)G, A(3222)T, T(3227)A | PmeIc | None |
| M gene 5′-NTRb | T(4452)G, T(4453)C, T(4457)C, A(4458)G, T(4459)C | AsiSIc | None |
| HN-L IGS | A(8327)C, G(8330)A | SnaBIc | None |
| L ORF | G(10396)A, A(13537)G | BstBIc | GAG to GAA, silent |
| MluIc | GCA to GCG, silent | ||
| P ORF | A(13537)G | RsrIId | CGG to CGA, silent |
a
Nucleotide changes are relative to the full-length antigenome nucleotide sequence and were the same in both strains. The GenBank accession number for GBT is GU978777. The BC genome sequence was first determined in our laboratory, as described in reference 10.
b
The 5′-NTR is the nontranslated gene region downstream of the respective ORF.
c
The restriction site was added to the antigenome.
d
The restriction site was removed from the antigenome.
e
The mutated nucleotide is shown in bold.