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Primers and probes for quantitative real-time RT-PCR
| Target (reference) | Sequence |
|---|---|
| IFN-α4 | |
| Forward | CAACTCTACTAGACTCATTCTGCAAT |
| Reverse | AGAGGAGGTTCCTGCATCACA |
| Probe | ACCTCCATCAGCAGCTCAATGACCTCAAA |
| IFN-β | |
| Forward | GCTCCTGGAGCAGCTGAATG |
| Reverse | TCCGTCATCTCCATAGGGATCT |
| Probe | TCAACCTCACCTACAGGGCGGACTTC |
| SARS-CoV N gene (56) | |
| Forward | AGGAACTGGCCCAGAAGCTT |
| Reverse | AACCCATACGATGCCTTCTTTG |
| Probe | ACTTCCCTACGGCGCTA |
| β-Actin | |
| Forward | ACGGCCAGGTCATCACTATTG |
| Reverse | CAAGAAGGAAGGCTGGAAAAGA |
| Probe | CAACGAGCGGTTCCGATGCCC |
