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Phylogenetic tree analysis of CSF and blood env sequences in subtype B R5 SHIVE macaques. Neighbor-joining phylogenetic trees of env V3-to-V5 sequences from the CSF and blood plasma rooted to HIV-1SF162 are shown, with bootstrap values of >70% indicated at the appropriate nodes. Genetic-distance scales representing the number of nucleotide substitutions per site between env sequences (0.005) are shown at the bottom of each tree. *, sequence obtained by conventional PCR.
