FIG 6.
CD4-independent Envs in the brains of subtype C SHIVE macaques GC98 and FI08. Env clones amplified from the cerebellum (blue) and occipital (purple) and parietal (green) regions and from the CSF (red) were assessed for the ability to enter Cf2th.CCR5 cells. Infectivity in these CD4-negative cells is expressed as a percentage of that of cells expressing both receptors. The dashed lines delineate 20% CD4-independent entry efficiency, and the insets show absolute RLU in Cf2Th.CD4hi.CCR5 cells of pseudovirions expressing representative Env populations with >20% CD4i entry efficiency. The data are the means and SD of 2 or 3 independent experiments.