TABLE 2.
Histopathologic lesions and staining characteristics in the brains of subtype B R5 SHIVSF162P3N-infected macaques with SHIVE
| Animal | Time to AIDS (wk) | Histopathologic lesion type(s)a | Immunohistochemical stainingb |
||
|---|---|---|---|---|---|
| SIVp27 (morphology) | CD68/CD163 | GFAP | |||
| DG07 | 7 | I | +/− | +/− | ND |
| T799 | 224 | I | + (Mϕ) | ++ | ++ |
| DE86 | 12 | I, II | ++ (Mϕ) | ++ | + |
| DG08 | 20 | I, II | +++ (Mϕ) | +++ | + |
| DN57 | 30 | I, II | +++ (Mϕ) | +++ | + |
| ET94 | 39 | I, II, III | ++++ (Mϕ; microglia) | +++ | ++ |
| DG17 | 22 | I, II, III | ++++ (Mϕ; microglia) | ++++ | ++ |
a
Three main types of lesions were observed: type I, perivascular histolytic with MNGCs; type II, severe parenchymal with white matter damage; type III, chronic burnt out.
b
Subjective semiquantitative scoring (from −, indicating no change over control brain sections, to ++++, indicating most severe) for the presence of SIVp27 and reactivity with the macrophage/microglia (CD68/CD163) and GFAP astrocyte marker by immunohistochemistry. Mϕ, macrophage; ND, not performed.