FIG 1.
Detection of aae-miR-2940 species in small RNA fractions from C6/36 A. albopictus cells. (A) Sequence and secondary structure of pre-aae-miR-2940 hairpin obtained from miRBase with the location of probes used for detection of aae-miR-2940-5p (5p-probe), aae-mir-2940-3p (3p-probe), and pre-aae-miR-2940 (pre-miRNA-probe). (B) Northern blot analysis with probes in panel A of small RNAs from C6/36 cells infected with WNVKUN at 3 and 5 dpi or mock infected. The experiments were repeated five times, and the blots shown are representative of these experiments. The levels of 5S rRNA and tRNA detected by ethidium bromide staining of the gels prior to transfer (lower panels) serve as loading controls and evidence for RNA integrity. M, mock; dpi, days postinfection; MW, molecular weight marker (GeneRuller Ultra-Low-Range DNA Ladder; Thermo Scientific).