Skip to main content
. 2014 Aug;88(15):8457–8467. doi: 10.1128/JVI.00317-14

FIG 3.

FIG 3

Effect of WNV subgenomic RNA (sfRNA) on aae-miR-2940-5p levels in C6/36 cells. (A) The predicted target of aae-miR-2940-5p in WNV 3′UTR and the seed region is underlined. (B) Mutated predicted target site of aae-miR-2940-5p in WNV 3′UTR. (C) Detection of FAM-labeled in vitro-transcribed sfRNA in transfected C6/36 cells. (D) Northern blot analysis of small RNAs from C6/36 cells transfected with in vitro-transcribed wild-type WNV sfRNA (WT) and sfRNA bearing a mutated aae-miR-2940 target site (Mut). RNA from cells transfected with in vitro-transcribed RNA complementary to sfRNA (NC) and from mock-transfected cells (M) serve as a negative control. A blot was probed with DNA probe complementary to aae-miR-2940-5p. In vitro transcription, transfection, and detection were repeated twice. The levels of 5S rRNA and tRNA detected by ethidium bromide staining of the gels prior to transfer (lower panel) serve as loading controls and evidence for RNA integrity.