FIG 5.
Effect of aae-miR-2940 mimic and inhibitor on WNV replication in infected C6/36 cells. Virus growth kinetics were assessed in C6/36 cells transfected with aae-miR-2940 mimic or inhibitors and infected with WNVKUN at an MOI of 1. Titer was determined by plaque assay on BHK-21 cells. WNV titer in culture fluids from cells transfected with nonspecific miRNA mimic and inhibitor were used for comparison. NS, nonspecific. Each transfection experiment was repeated three times, and plaque assays were performed in duplicate for each sample. Data on the graph are means of triplicates, with error bars indicating minimum and maximum values. Statistical analysis was performed using two-way ANOVA (***, P < 0.001; **, P < 0.01; *, P < 0.1).