FIG 6.

Analysis of plaque and pock sizes of mutant viruses. (A) Relative plaque sizes. Diameters of 75 plaques of each construct were measured in three independent experiments. Plaque diameters of all repaired viruses were not different from those of vBRFseR (data not shown). (B) Relative pock sizes. Diameters of 25 pocks of each of the viruses were measured. Plaque sizes and pock sizes were analyzed by analysis of variance for multiple comparisons with Bonferroni corrections. Significance is indicated as follows: *, significantly different from vBRFseR with an alpha of 5%; **, highly significant difference from the wild type with an alpha of 1%; ns, not significantly different from vBRFseR. (C) Correlation between plaque and pock sizes. The average relative sizes of plaques and pocks were analyzed by a nonparametric correlation (Spearman) test. Mutants with the same reductions in plaque and pock sizes are located on the diagonal. Mutants with greater reductions in pock sizes are above the diagonal. Mutants below the diagonal have greater reductions in plaque sizes. Three different symbols were used to represent different types of pocks. Abbreviated names of viruses are used in the figure (wt for parental vBRFseR, dcrmA for deletion of crmA, and d060 for vBRFseRd060, where d060 indicates the deletion of CPXV060, etc.).