TABLE 3.
Primers used for cloning
| Name | Sequence (5′–3′) | Description of amplified sequence |
|---|---|---|
| I-SceIF | AAACTAGGGATAACAGGGTAATCTCATGTTTGACAGCTTATCATC | Tetracycline cassette, adding I-SceI restriction site |
| I-SceIR | AAGAATTGATTGGCTCCAATTC | |
| BR060F | GACTTTACACAACGCGATATATAACTTTC | Part of ORF CPXV060 |
| BR060R | GACGACGAATATGTTCATATCACTTC | |
| BR064F | GCTGTTTCGAAACTTTATCAATACAC | Part of ORF CPXV064 |
| BR064R | GCAGGGGTATTAATATCAGGGTATC | |
| BR068F | TCGCATATATTCCCAAGTCTTCAGT | Part of ORF CPXV068 |
| BR068R | GCTGTAGATACACTTCTTGGTTACG | |
| BR069F | CGGAATAACATCATCGAAAGAC | Part of ORF CPXV069 |
| BR069R | GGGTTAATCAGAGCCACATTC | |
| BR074F | ACATAGTTGATAAAAAGCGGTAGG | Part of ORF CPXV074 |
| BR074R | TACCGGAGAAAGATCCATTAGC | |
| BR136F | GTTGTGCTGTTGTACATACTGTACC | Part of ORF CPXV136 |
| BR136R | AATGGACTTCTTTAACAAGTTCTCAC | |
| BR168F | CTAAGGTCGTTAGTAGGGAGGC | Part of ORF CPXV168 |
| BR168R | GCATCCGAGAATGACTTGTAGTC | |
| BR169F | CGATGTCTTGACTACCTGGCTC | Part of ORF CPXV169 |
| BR169R | GAATCCGTCGTACTGTTTAGTTG | |
| BR172F | CTTCATTCTGTATATCAGACGGC | Part of ORF CPXV172 |
| BR172R | CCACAACATTGGATTCGTTATC | |
| BR199F | GAGATAAATGGTCGTGTTTTCC | Part of ORF CPXV199 |
| BR199R | CACGTAACAGTATCATTCCAAGAAG | |
| BR207F | TGAGTGGTGGTAGTTACGGATATC | Part of ORF CPXV207 |
| BR207R | AGTATCTCCAACATATGGCAGTTC |