TABLE 6.

CPXV genes responsible for hemorrhagic phenotype, their functions, and homologue similarity

Group and CPXV-BR ORF	Protein length (aa)f	VACV-COP homologue(s)	Function(s)a	Pock type of mutantb	Actin tail induction	EEV productionc	Conservationd	Similarity (%) of protein homologues in different OPVS
								CPXV-GRI	VACV-Lister	VACV-COP	VACV-MVA	VARV	MPXV	RPXV
Immune suppressor
CPXV069	190	E3L	Interferon resistance and PKR inhibitor	II	No	No	O	94.74	96.32	96.32	96.32	96.84	90.85	95.79
CPXV207 (crmA)	341	B13R/B14Re	Serine Protease Inhibitor-2	I	No	No	O	94.48	96.33	96.79	95.45	94.77	94.77	95.06
Core protein
CPXV074	273	E8R	Endoplasmic reticulum localized membrane protein	I	No	No	C	99.63	99.27	99.27	99.27	98.90	99.27	99.27
CPXV136	295	A4L	Core protein	I	No	No	C	92.2	90.85	91.19	88.81	87.50	89.49	91.86
EEV-related protein
CPXV068	737	E2L	IEV- associated protein	II	No	Yes*	C	99.32	99.32	99.32	99.32	99.05	98.91	99.46
CPXV060	634	F12L	IEV- associated protein	III	Yes	Yes*	C	98.42	98.11	98.11	98.27	97.01	98.43	98.27
CPXV168	187	A33R	EEV glycoprotein	III	Yes	Yes†	O	98.40	97.33	97.33	97.33	93.58	97.25	97.33
CPXV169	168	A34R	EEV C-type lectin-like protein	III	Yes	Yes†	O	100.00	100.00	99.40	99.40	100.00	100.00	100.00
CPXV172	224	A36R	IEV specific	III	Yes	Yes*	O	97.78	96.89	96.89	93.18	93.78	95.11	96.89
CPXV199	317	B5R	EEV complement control protein	III	Yes	Yes*	O	97.48	97.74	96.85	97.16	97.47	97.79	96.85
Unknown
CPXV064	477	F15L	Unknown	II	?	?	C	98.10	98.10	97.47	97.47	97.39	97.47	97.47

^aInformation about functions were based on studies of homologues in vaccinia virus and obtained from www.poxvirus.org.

^bTypes of pocks were determined according to results of fluorescence microscopy (Fig. 3).

^cNo, not involved in the production of EEV; yes, involved in the production of EEV; *, deletion mutant produces less EEV than the wild type; †, deletion mutant produces more EEV than the wild type (23).

^dC, gene conserved in chordopoxviruses (16); O, gene conserved only in orthopoxviruses (www.poxvirus.org).

^eB13R/B14R, a homologue of the CPXV207 (crmA) gene in VACV, is not functional due to formation of a premature stop codon.

^faa, amino acids.