TABLE 1.

Primer sequences used

Primera	Sequenceb	Name
1	5′CAACTCTCCAAGCGGCAATC	C5-M F
2	5′AGCGAGAGAGGTAACGATTAGTTTTTTGTGTC	C5-M R
3	5′atagttgtagccaccATGGTGAGCAAGCAGA	GFP F
4	5′acggtagttacacacTCACACCCACTCGTG	GFP R
5	5′CCGCTTGGAGAGTTGGACCTTG	C5 M vec Up
6	5′GTTACCTCTCTCGCTTCCTCAG	LS M vec Down
7	5′GGTGGCTACAACTATCAACTAAACT	Insert vec Up
8	5′GTGTGTAACTACCGTGTACTAAGC	Insert vec Down
9	5′atagttgtagccaccATGCAATCCTACATCG	Plant-gB/GFP F
10	5′gtagttacacacagcTTATTCGTCTTCGCTTTC	Plant-gB/GFP R
11	5′atagttgtagccaccATGCACCGTCCTCATC	Plant-gD/GFP F
12	5′gtagttacacacagcTTAGCTACGCGCGCAT	Plant-gD/GFP R

^aPrimers 1 and 2 were used to PCR amplify a cDNA fragment spanning the LaSota P and M gene junction region, primers 3 and 4 were used to PCR amplify a cDNA fragment containing the GFP gene ORF, primers 5 and 6 were used to PCR amplify or linearize the pFLC-LaSota vector in the noncoding region of the M gene end region, primers 7 and 8 were used to amplify or linearize the pT-LS-M, pT-LS-GFP, and pLS-GFP vectors, primers 9 and 10 were used to amplify a cDNA fragment containing the ILTV gB gene ORF, and primers 11 and 12 were used to amplify a cDNA fragment containing the ILTV gD gene ORF.

^bNucleotides shown in lowercase letters represent homology sequences with a vector backbone, which were used to facilitate the restriction endonuclease site (RE)-independent cloning using the In-Fusion PCR cloning kit (Clontech).