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TEM images of negative stained EV1. (A) Intact EV1 virion. (B) EV1 heated at 50°C for 3 min shows an electron-dense protrusion from an otherwise structured particle. (C) Heating for 10 min at 60°C results in complete particle disruption. Scale bars, 25 nm and 100 nm (left and right images, respectively).
