TABLE 1.
Assignmenta | Frequency (cm−1) |
Heat-induced effect | |
---|---|---|---|
Dried sample | Aqueous sample | ||
Phe | 623 | 620 | + |
Tyr | 644 | 642 | |
C-S | 670 | 668 | |
Trp | 758 | 756 | + |
Tyr | 826, 855 | 829, 854 | − (829)/+ (854) |
Trp | 887 | 873–883 | − (60°C) |
AmIII | 960–978 | 952–977 | |
Phe | 1,003 | 1,003 | |
Phe | 1,031 | 1,031 | |
CC, CH | 1,071-1,096 | 1,073-1,098 | + |
Nonaromatic side chains | 1,127 | 1,122-1,128 | + (1,120)/− (1,130) |
CH3 | 1,156-1,176 | 1,160-1,178 | |
Phe, Tyr | 1,208 | 1,205-1,210 | |
Am III | 1,230-1,300 | 1,230-1,300 | + (1,240)/− (1,300) |
Trp, CH3 | 1,318-1,378 | 1,322-1,383 | |
Asp, Glu-COO− | 1,390-1,404 | 1,390-1,402 | + (60°C) |
CH2 | 1,446-1,458 | 1,446-1,458 | − |
Trp | 1,555, 1573 | 1,549, 1,572 | + |
Phe | 1,588-1,594 | − | |
Phe, Trp | 1,605 | 1,603 | |
Tyr, Trp | 1,613, 1,618 | 1,612, 1,616 | |
Am I | 1,669 | 1,668 | + |
Asp, Glu-COOH | 1,710 | + (50°C) |