TABLE 1.
Primary antibodies used in dual immunofluorescence
| Antigen and/or sample type | Host species | Source (location) | Concn | Name or catalog no. |
|---|---|---|---|---|
| Prion protein | ||||
| Tongue | Mouse | Rocky Mountain Laboratories, (Hamilton, MT) | 1:6,000 | 3F4a |
| dCAD cells | Mouse | D-Gen (London, UK) | 1 μg/ml | ICSM 33 |
| Synaptophysin | Rabbit | Epitomics (Burlingame, CA) | 1 μg/ml | 1485-S |
| βIII-Tubulin | Rabbit | Covance Research Products (USA) | 5 μg/ml | PRB-435P |
| LAMP-1 | Rabbit | Abcam (Cambridge, MA) | 2.25 μg/ml | ab24170 |
| Cathepsin D | ||||
| Tongue | Rabbit | Dako (Carpinteria, CA) | 5.8 μg/ml | A0561 |
| dCAD cells | Rabbit | Santa Cruz Biotechnology (Santa Cruz, CA) | 0.5 μg/ml | sc-10725 |
| Myelin P2 | Goat | Santa Cruz Biotechnology (Santa Cruz, CA) | 1 μg/ml | sc-49304 |
a
See reference 60.