TABLE 3.

Colocalization analysis of PrP^Sc and cellular markers in nerve bundles and neuron soma in the tongue following HY TME inoculation

Cellular maker and/or cell type	Sampling date (no. of wks p.i.)a	Nerve bundle MOCb	Neuronal soma (ganglion) MOCb	Cellular structure
βIII-Tubulin	8–9	NS	ND	Microtubule network in axons
Synaptophysin	8–9	NS	ND	SVPs in axonsd
LAMP-1	8–9	**	*	Late endosomes, lysosomes
	13	***	***
dCAD cellsc		***	***
Cathepsin D	13	*	*	Mature lysosomes
Myelin P2	8–9	*	ND	Myelin

^ap.i., postinfection.

^bA t test (unpaired, two-tailed) comparing the Manders' overlap coefficient (MOC) of PrP^Sc and cellular markers in mock-infected and HY TME-infected tongue or between mock-infected and 22L scrapie-infected differentiated CAD cells in vitro was performed. ND, not done; NS, not statistically significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

^cdCAD, CAD cell line was switched to serum-free medium to induce differentiation, which results in cessation of cell division, extension of neurites, and formation of phase-bright cell bodies. For dCAD cells, LSCM analysis was performed in neurites and cell bodies and could be morphologically related to axons in nerve bundles and somata of ganglia cells, respectively, in the tongue.

^dSVPs, synaptic vesicle precursors.